Derbyshire and Gray Laboratory Gallery
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ESX-1 primarily associates with the old “non-septal” pole. M. smegmatis cells expressing a fusion of msmeg0046::yfp were imaged every 10 mins in a heated growth chamber. The fluorescent fusion protein (red) localizes to a single cell pole, the old pole (Wirth et al., Mol. Micro. 2012).
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Transconjugant colonies with different morphotypes result from a cross between M. smegmatis strains MKD24 and Jucho
A transconjugant morphotype; colonies of a purified transconjugant isolated from a cross between M. smegmatis strains MKD24 and Jucho
A transconjugant morphotype; colonies of a purified transconjugant isolated from a cross between M. smegmatis strains MKD24 and Jucho
![An M. smegmatis biofilm containing donor cells, mc2155 An M. smegmatis biofilm containing donor cells, mc2155](/sites/default/files/program_galleries/Fig_4.jpg)
An M. smegmatis biofilm containing donor cells, mc2155
![A biofilm containing both donor and recipient cells of M. smegmatis A biofilm containing both donor and recipient cells of M. smegmatis](/sites/default/files/program_galleries/Fig_5.jpg)
A biofilm containing both donor and recipient cells of M. smegmatis
![A fluorescent image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra. A fluorescent image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dend](/sites/default/files/program_galleries/Fig_6a.jpg)
A fluorescent image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra.
![A phase contrast image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra A phase contrast image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein d](/sites/default/files/program_galleries/Fig_6b.jpg)
A phase contrast image of the edge of an M. smegmatis colony expressing a mycobacterial gene fusion to the fluorescent protein dendra
![Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact. Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain c](/sites/default/files/program_galleries/Fig_7.jpg)
Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact.
![Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact. Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain c](/sites/default/files/program_galleries/Fig_8.jpg)
Fluorescent image of a mixture of donor (blue) and recipient (red) M. smegmatis cells grown in coculture. The recipient strain contacts a gfp reporter, which is activated (green) when donor and recipient cells are in direct cell contact.