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Research & Development

Since 1995, the Bacteriology Laboratory has been developing diagnostic molecular assays to supplement the culture-based testing available.

The assays are for public health purposes and are rarely commercially available. The tests used include real-time PCR and various DNA sequencing methods and analysis.

Also of interest are molecular diagnostic assays that detect the serotype or characterize bacteria to determine if genes that confer pathogenicity, toxin genes, or mobile genetic elements such as plasmids or transposons that associated with antibiotic resistance are present. 

Research Projects

The Bacteriology Laboratory has developed many molecular based assays to rapidly detect and/or identify/characterize bacterial pathogens.

  • Development of symptom-based molecular testing panels
  • Development of whole-genome sequence (WGS) based methods for strain typing and diagnostic characterization including Mycobacterium tuberculosis as well as foodborne, waterborne and healthcare associated bacterial pathogens.
  • Implementation of a national surveillance system for foodborne diseases based on WGS in collaboration with the CDC and FDA.
  • Discovery and characterization of new species of bacteria recovered from New York State patients.
  • Evaluation of MALDI-TOF MS technology 
  • Characterization and maintenance of a historic bacterial culture collection 
  • Evaluation of commercially available tests and other methodologies
  • Development of real-time PCR assays for newly emerged pathogens
  • Development of novel diagnostic real-time PCR assays  
  • Development and optimization of direct specimen (culture independent) next generation sequencing (NGS) approaches
  • Research into the diversity of bacterial strains within isolates from clinical specimens
  • Research into novel antimicrobial resistant bacteria
  • Evaluation of NGS and WGS approaches for understanding the spread of antimicrobial resistance
  • Creation of a database of circulating transmissible plasmids conferring antimicrobial resistance in New York.
  • Bioinformatic pipeline development for bacterial pathogens including Legionella, foodborne bacteria, antimicrobial resistant bacteria

Funding

The Bacteriology laboratory is also one of ten public health laboratories in the country that participate in the Emerging Infections Program (EIP), a national program funded by the Centers for Disease Control and Prevention (CDC) to establish and strengthen surveillance for new and emerging infections, and to develop prevention and control measures. The laboratory components of the EIP include The Foodborne Diseases Active Surveillance Network (FoodNet), Active Bacterial Core surveillance (ABCs), and Healthcare Associated Infections-Community Interface (HAIC) projects.

 
The Bacteriology Laboratory participates in several U.S Food & Drug Administration (FDA) funded programs including the The National Antimicrobial Resistance Monitoring System (NARMS) including the Retail Meat Study grant and the GenomeTrakr network. These programs allow our laboratory the ability to contribute to the understanding of foodborne bacteria in retail meats and environmental sampling along with other public health labs and university partners to improve available genomic and geographic data and improve outbreak investigations.

The Bacteriology Laboratory has been a recipient of the Epidemiology and Laboratory Capacity for Prevention and Control of Emerging Infectious Diseases (ELC) Cooperative Agreement since 1996. This funding has been critical to U.S. health departments’ ability to combat infectious diseases and has had a major impact on our laboratory’s ability to detect, respond to, control, and prevent infectious diseases in New York. We continue to have funded projects in the following areas- Laboratory Capacity, PulseNet, PulseNet Area laboratory, NARMS, Antibiotic Resistance Laboratory Network (AR Lab Network).
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The Bacteriology Laboratory contributes to the Wadsworth Center’s role announced in the initiation of a collaborative relationship with Merck's ILÚM Health Solutions (now IDC) and OpGen to develop a state-of-the-art research program to detect, track, and manage antimicrobial-resistant infections at healthcare institutions statewide. Multiple offices within the Department are participating in this public-private partnership, which has been facilitated by the Governor’s Life Sciences initiative.

Related press releases:

The Bacteriology has several long-term contracts through the CDC and Association of Public Health Laboratories (APHL) focused on the improvement of new technologies for including whole-genome sequencing (WGS) and culture independent WGS for Legionella and Mycobacterium tuberculosis. These have included the following Request for Proposals (RFPs):

 

 

Bacteriology

  • Banaei N, Musser KA, Salfinger M, Somoskovi A, Zelazny AM. Novel Assays/Applications for Patients Suspected of Mycobacterial Diseases. Clin Lab Med. 2020;40(4):535-552.10.1016/j.cll.2020.08.010
  • Pubmed Web Address

  • Shea J, Halse TA, Kohlerschmidt D, Lapierre P, Modestil HA, Kearns CH, Dworkin FF, Rakeman JL, Escuyer V, Musser KA. Low-level rifampin resistance and rpoB mutations in Mycobacterium tuberculosis: An analysis of whole-genome sequencing and drug susceptibility test data in New York. J Clin Microbiol. 2020;Sept 30(JCM):01885-20.10.1128/JCM.01885-20
  • Pubmed Web Address

  • Smith C, Halse TA, Shea J, Modestil H, Fowler RC, Musser KA, Escuyer V, Lapierre P. Assessing Nanopore sequencing for clinical diagnostics: A comparison of NGS methods for Mycobacterium tuberculosis. J Clin Microbiol. 2020;Oct 14(JCM):00583-20.10.1128/JCM.00583-20
  • Pubmed Web Address

  • Prussing C, Snavely EA, Singh N, Lapierre P, Lasek-Nesselquist E, Mitchell K, Haas W, Owsiak R, Nazarian E, Musser KA. Nanopore MinION Sequencing Reveals Possible Transfer of bla KPC-2 Plasmid Across Bacterial Species in Two Healthcare Facilities. Front Microbiol. 2020;August 19(11):2007.10.3389/fmicb.2020.02007
  • Pubmed Web Address

  • Ramautar AE, Halse TA, Arakaki L, Antwi M, Del Rosso P, Dorsinville M, Nazarian E, Steiner-Sichel L, Lee L, Dickinson M, Wroblewski D, Dumas N, Musser K, Isaac B, Rakeman J, Weiss D. Direct molecular testing to assess the incidence of meningococcal and other bacterial causes of meningitis among persons reported with unspecified bacterial meningitis. Diagn Microbiol Infect Dis. 2015;83(3):305-11.
  • Pubmed Web Address